NAD+

Each lot of NAD+ is analytically characterized to support its use as a research reagent:

• Purity: High-performance liquid chromatography (HPLC) verified to ≥99%.
• Identity: Confirmed by mass spectrometry (MS) against the expected molecular ion.
• Reference identifiers: CAS 53-84-9; molecular formula C₂₁H₂₇N₇O₁₄P₂; molecular weight ~663.4 g/mol — lot-specific values reported on the COA.
• Certificate of Analysis (COA): A lot-specific COA documenting the analytical results is available for each batch.

These figures are analytical characterizations of identity and purity only and do not describe biological activity or suitability for any use in humans or animals. For research use only, not for human use.

A Certificate of Analysis is available per production lot, documenting HPLC purity and mass-spectrometry identity confirmation.

View Certificates of Analysis →

For Research Use Only · Not for human or veterinary use · Not for diagnostic or therapeutic use.

References to clinical phases/studies describe the compound in independent third-party literature (e.g., ClinicalTrials.gov, PubMed) and do not represent claims, testing, or approval by Zynix Biolab.

NAD+ (β-nicotinamide adenine dinucleotide) is a naturally occurring pyridine dinucleotide coenzyme, not a peptide. It is built from two nucleotides — one bearing a nicotinamide moiety and the other an adenine moiety — joined through their phosphate groups. In the published literature it is characterized as a central redox cofactor (the NAD+/NADH couple) in cellular respiration and energy metabolism, and as a co-substrate consumed by NAD+-dependent enzymes such as the sirtuins, poly(ADP-ribose) polymerases (PARPs) and CD38. Reported molecular formula C₂₁H₂₇N₇O₁₄P₂; reported molecular weight ~663.4 g/mol; CAS 53-84-9 — values as characterized on the lot-specific COA.

In third-party scientific literature, NAD+ metabolism has been extensively reviewed in the context of cellular processes during ageing, including mitochondrial function, DNA repair, chromatin regulation and cellular senescence (Covarrubias et al., Nature Reviews Molecular Cell Biology, 2021). NAD+ biology has also been examined in registered and published human studies of NAD+ precursors — for example, a placebo-controlled, randomized, double-blind trial of the precursor nicotinamide mononucleotide reported elevated blood NAD+ levels in older participants (Igarashi et al., npj Aging, 2022). These references describe independent academic research on the compound and its metabolism and do not represent testing, validation, or approval of this product by Zynix Biolab. This material is supplied for research use only and is not for human use.

For Research Use Only · Not for human or veterinary use · Not for diagnostic or therapeutic use.

In the published literature, NAD+ has been investigated and used as a reagent and study subject across in vitro and laboratory research lines, including:

• As a redox cofactor (NAD+/NADH) in studies of cellular respiration, glycolysis and oxidative phosphorylation in cell-free and cell-culture systems.
• As a co-substrate in biochemical characterization of NAD+-consuming enzymes, including sirtuins, PARPs and CD38.
• In laboratory research into mitochondrial metabolism, DNA-repair pathways and cellular senescence at the cell and tissue-model level.
• As a standard or substrate in enzyme-kinetics and metabolomics assays examining NAD+ metabolism and its decline in ageing models.

These descriptions are provided for scientific context only and summarize uses reported in third-party literature. This product is not for human use, diagnostic use, or therapeutic use.

General laboratory handling guidance for the research reagent:

• Storage: Store the lyophilized powder at -20°C, protected from light and moisture.
• Reconstitution: For laboratory preparation, reconstitute with bacteriostatic water or another suitable research-grade solvent; NAD+ is light- and pH-sensitive, so prepare working solutions fresh and keep on ice during use.
• Aliquoting: Aliquot reconstituted material to minimize freeze-thaw cycles.
• PPE: Handle with appropriate personal protective equipment (gloves, lab coat, eye protection) under standard laboratory safety practices.

This guidance describes laboratory handling of a research material and is not a dosing or administration instruction. For research use only — not for human use.

For Research Use Only · Not for human or veterinary use · Not for diagnostic or therapeutic use.

• Covarrubias AJ, Perrone R, Grozio A, Verdin E. NAD+ metabolism and its roles in cellular processes during ageing. Nature Reviews Molecular Cell Biology. 2021;22(2):119-141. PMID: 33353981. https://doi.org/10.1038/s41580-020-00313-x
• Igarashi M, Nakagawa-Nagahama Y, Miura M, et al. Chronic nicotinamide mononucleotide supplementation elevates blood nicotinamide adenine dinucleotide levels and alters muscle function in healthy older men. npj Aging. 2022;8:5. https://doi.org/10.1038/s41514-022-00084-z · PMC9158788

These references are provided for scientific context only and are not claims by Zynix Biolab.